Monday, November 1, 2010

Cell disruption / breakage for protein release

•Extraction techniques are selected based on the source of protein (e.g. bacteria, plant, mammalian, intracellular or extra cellular)
•Use procedures that are as gentle as possible. Cell disruption leads to the release of proteolytic enzymes and general acidification
•Selection of an extraction technique often depends on the equipment availability and the scale of operation
•Extractions should be performed quickly, at sub-ambient temperatures in a suitable buffer to maintain pH and ionic strength
•Samples should be clear and free of particles before beginning chromatography

No comments:

Post a Comment