1 Chemical / enzymatic
•Cell lysis (osmotic shock and freeze thaw)
•Enzymatic digestion
Blood cells
Mammalian cells
•Fractional precipitation
•Extra cellular proteins
2 Mechanical
•Hand and blade homogenizers
tissue
•Sonicator / disruptors
•Grinding with abrasive
plant/yeast
•Bad beaters / mill
•French press
•micro fluidizer
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