Methods for quantification of proteins in solution

Assay method Useful range Comments

NanoOrange assay 100ng/ml to 10ug/ml ·Samples can be read up to six
hours later without any loss in
the sensitivity

·Low protein to protein signal
variability

·Detection not influenced by
reducing agents or nucleic acid

BCA method
(Cu reduction) 0.5ug/ml to 1.5mg.ml ·Samples must be read within
10mins

·Not compatible with reducing
agents