Protein purification

•Affinity chromatography
–Binding to immobilised ligands e.g antibodies, co-factors
•Ion exchange chromatography
–Anion (-) and cation (+) exchanger
•Hydrophobic interaction chromatography
–Colum coated with hydrophobic fatty acid chains
•Size exclusion chromatography
–Gel filtration
•Electrophoresis
–SDS